TGR5 (Gpbar-1) mediates cholangiocyte proliferation via ROS-Src-EGFR-ERK1/2 signaling

Introduction: TGR5 (Gpbar-1) is a membrane-bound bile acid receptor, which is expressed in cholangiocytes. In biliary epithelial cells the activation of TGR5 via bile acids promotes chloride secretion, proliferation and protects the cells from apoptosis (Xia et al., WJG 2006; 12:3553 – 3563). Aim of this study was to determine the role of TGR5 for cholangiocyte proliferation and to elucidate the underlying signalling mechanisms. Methods: Cholangiocytes were isolated from TGR5 knockout and wildtype mice. Cell proliferation was measured using a BrdU incorporation assay. Protein expression was investigated by weste…