Humoral factors by human bone marrow-derived MSC enhance beta cell proliferation via ERK1/2 signalling
C Liu, L Lang, AE Mehana, B Mihic-Necic, L Parsons, J Baumann, C Jäger, N Perakakis, K Laubner, J Seufert, G Päth · Diabetologie und Stoffwechsel · 2014 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and aim: We previously demonstrated that human telomerase-immortalised MSC (hMSC-TERT) migrate towards injured rat INS-1E beta cells, promote their survival and reduce their apoptosis via p-AKT-Bcl2/Bax signalling pathway. Both cell lines are valid models since they closely resemble results from primary rat MSC and rat islets. Here, we further tested whether hMSC-TERT promote the proliferation and function of INS-1E, and investigated the underlying mechanism. Methods: Cocultures were performed by inserts with 0.4 µm pores to allow soluble factors but not cells to pass the membrane. Proliferation was a…
