Three-dimensional reconstruction and quantification of key features of liver microarchitecture

Liver disease is often associated with altered tissue microarchitecture. However, robust techniques for immunostaining, 3D reconstruction and quantification still represent a bottleneck. To bridge this gap, we have established protocols of antibody combinations for immunostaining, confocal imaging, three-dimensional reconstruction of 100 µm thick tissue blocks, and quantification of key architectural features in the liver. For the simultaneous visualization of bile canaliculi, sinusoidal endothelial cells and central veins as well as DNA, dipeptidyl-peptidase IV/CD26, donkey anti-mouse IgG, glutamine synthetase…