Comprehensive analysis of informative target disease relevant nuclear genes and the whole mitochondrial genome in hepatocellular carcinoma by Multiplex PCR and Ultra-Deep Sequenci…

Hepatocellular carcinoma (HCC) are highly malignant tumors triggered by a wide variety of genetic alterations. Importantly, they show multicentric occurrence characterized by a nodule-in-nodule pattern. In the present study, we investigate the occurrence of disease related nuclear mutations and whole mitochondrial genomic variants by a comprehensive ultra-deep sequencing approach as a novel tool for hepatocellular cancer follow-up and prognosis. Macrodissected 48 HCC nodules, representing different tumor grades, and adjacent non-cancerous tissues from HCC patients were studied. Hot spot mutation screening of nuc…