Delta like ligand4 modulates chemokine ligand 2 through impacting the NFkB pathway

Introduction: Our previous study showed that recombinant Delta like ligand 4 (rDll4) attenuated liver damage in carbon tetrachloride challenged mice through inhibiting chemokines, particularly chemokine ligand 2 (Ccl2). This study investigated the role of rDll4 in mice with bile duct ligation (BDL) and mechanisms how Dll4 may regulate Ccl2. Methods: The function of Dll4 was examined in BDL-challenged mice. The effect of Dll4 on LPS-induced-Ccl2 expression was investigated in macrophages (RAW264.7 cells) using qPCR, immunoblot, and Ccl2 promoter reporter assays. Results: In BDL mice, rDll4 induced massive hepatic…