Deficiency of Group VIA phospholipase A2 Primes the Cytokine Releases by Kupffer cells and Lymphocytes

Background and Aims: Impairment of apoptotic cell clearance has been implicated in autoimmunity. We have observed this impairment upon iPLA2β deficiency causing accumulation of apoptotic cells in liver and intestine, and iPLA2β-/- mice were susceptible for injury induced by concanavalinA (ConA) or dextran-sodium-sulfate. Here we aim to determine whether iPLA2β deficiency or combined with CD95/FasL ligation (Jo-2) could alter cytokine release by immune cells. Methods: Kupffer cells (KC) or splenocytes and liver lymphocytes were isolated from WT and iPLA2β-/- 3-month-old male mice, and were stimulated by 1 µg/mL L…