Hyperthermia-driven aberrations of secreted microRNAs in breast cancer in vitro

Purpose: Expression profile alterations of nine breast cancer (BC) associated secreted microRNAs (miRs) were determined under microenvironmental alterations occurring in tumor progression, metastasis or specific oncological treatment modalities. Thereto, the potential influence of the exogenic stimuli hypoxia, acidosis and hyperthermia was investigated in vitro. Material and methods: Four established BC cell lines were applied as in vitro BC model systems. Quantitative analyses of secreted microRNA specimen were performed by RNA isolation from cell culture supernatant and subsequent realtime PCR in cells under p…