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Sonoporation by microbubbles as gene therapy approach for liver cancer

L Rinaldi, G Franci, V Folliero, L Palomba, R Isticato, C Zannella, R Di Francia, I De Sio, LE Adinolfi, A Ascione, G Morelli, S Lastoria, L Altucci, C Pedone, M Galdiero · Ultraschall in der Medizin - European Journal of Ultrasound · 2016 · Heft 08

Dokument
989059
CareLit-ID
Jahr
2016
Publikation
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Ultraschall in der Medizin - European Journal of Ultrasound
Autor:innen
L Rinaldi, G Franci, V Folliero, L Palomba, R Isticato, C Zannella, R Di Francia, I De Sio, LE Adinolfi, A Ascione, G Morelli, S Lastoria, L Altucci, C Pedone, M Galdiero
Ausgabe
Heft 08 / 2016
Seiten
–
Erschienen: 2016-08-18 00:00:00
ISSN
0172-4614
DOI
10.1055/s-0036-1587823

Zusammenfassung

Purpose: We use an innovative method, known as sonoporation, to induce the expression of silenced gene in liver cancer cells (HepG2), such as (but nit restricted to) TRAIL in a specific manner. Aim of the project is the re-activation of silenced apoptotic pathway in liver cancer models, using diagnostic microbubble synovial as plasmidic gene delivery. Material and methods: HepG2 ATCC were used to assess all the experiments. Microbubble (Sonovue®) were used at standard condition according to manufacturer's instructions. pEGFP-TRAIL plasmid (Plasmid #10953 addgene) and the respective control were selected and prop…

Schlagworte

Ultraschall in der Medizin - European Journal of Ultrasound